Detection of organophosphorus compound based on a sol–gel silica planar waveguide doped with a green fluorescent protein and an organophosphorus hydrolase

Organophosphorus hydrolase multilayer modified microcantilevers for organophosphorus detection.

We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10(-7) to 10(-3)M. The l...

Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase.

As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...

Organophosphorus Compound Induced 'Intermediate Syndrome'

Organophosphorus hydrolase-based assay for organophosphate pesticides

We report a rapid and versatile organophosphorus hydrolase (OPH)-based method for measurement of organophosphates. This assay is based on a substrate-dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which ...

a review on engineering of organophosphorus hydrolase (oph) enzyme

organophosphorus chemicals are compounds which have been used as pesticides and insecticides in agriculture. they’re also used as nervous agents and have raised many problems for human and environment. among the most important methods of decontamination from these compounds are biodegradation methods. using oph enzyme in degradation the mentioned compounds is seen as one of the desirable ways, ...